Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Clustering heatmap of microarray data showing differential expression of circRNAs between malignant, benign and normal cell lines. Unsupervised clustering (euclidean distance measure and the “average” agglomeration method) was used for analysis ( n = 3). circRNAs were more likely to be down-regulated in normal and benign cell lines compared to malignant cells.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques: Microarray, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 down-regulated circRNAs in PCa (malignant vs. normal/benign cell lines)*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Clustering heatmap showing differential expression of circRNAs between AR dependent cells LNCaP, 22Rv1 and VCaP (hormone sensitive) and AR independent cells DU145 and PC-3 (castration resistant). Unsupervised clustering (euclidean distance measure and the “average” agglomeration method) was used for analysis ( n = 3).

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 up-regulated circRNAs in androgen dependent vs. independent cell lines*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Differential CircRNA Expression Signatures May Serve as Potential Novel Biomarkers in Prostate Cancer

doi: 10.3389/fcell.2021.605686

Figure Lengend Snippet: Top 10 down-regulated circRNAs in androgen dependent vs. independent cell lines*.

Article Snippet: The human circular RNA microarray (Arraystar) version 2.0 covers 13,617 previously discovered human circRNAs.

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Circ-CAMTA1 regulated by Ca 2+ influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosus

doi: 10.1186/s13075-024-03422-6

Figure Lengend Snippet: Differential expression of circRNA in Jurkat cells after coculture with ionomycin (1 µg/mL) for 1 h. ( A ) The expression profiles of circRNA in Jurkat cells after coculture with ionomycin (1 µg/mL) for 1 h were evaluated using next-generation sequencing. Each scatter point in the figure represents the mean read count of circRNA in three replicates of the treatment. ( B ) Validation of potential candidates of Ca 2+ influx-regulated circRNAs in Jurkat cells. The relative expression level of circRNA was defined as (39 - Ct) after adjusted with the internal control (GAPDH; Glyceraldehyde-3-Phosphate Dehydrogenase). Data was presented as mean ± SD. (ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: To analyzed circRNA expression levels in Jurkat cells after stimulation, cells were treated with phorbol 12-myristate 13-acetate (PMA; 20 ng/mL; Sigma-Aldrich) and ionomycin (500 ng/mL; Sigma-Aldrich, ) for 24 h. For analysis of circRNA expression in response to interferon-alpha (IFN-α), Jurkat cells were treated with 1000 IU/mL recombinant human IFN-α for 72 h. The IFN-alpha we used was purchased from Proteintech (Rosemont, IL, USA) and was produced in HEK293.

Techniques: Quantitative Proteomics, Expressing, Next-Generation Sequencing, Biomarker Discovery, Control

Journal: Arthritis Research & Therapy

Article Title: Circ-CAMTA1 regulated by Ca 2+ influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosus

doi: 10.1186/s13075-024-03422-6

Figure Lengend Snippet: Differential expression of Ca 2+ influx-regulated circRNA in T cells from patients with SLE and controls and its association with SLE disease activity ( A ) The expression of the seven Ca 2+ influx-regulated circRNAs in T cells from patients with SLE and controls. The relative expression level of miRNA was defined as 39 - Ct, adjusted with the internal control. The expression levels of circ-CAMTA1, circ-ASH1L, and circ-ASAP1 were significantly lower in T cells from patients with SLE. After adjusting for age and sex, the SLE T cells still had lower expression levels of circ-CAMTA1 ( P = 0.008), circ-ASH1L ( P = 0.008), and circ-ASAP1 ( P = 0.046). ( B ) The expression levels of circ-CAMTA1, circ-ASH1L, and circ-ASAP1 in T cells from patients with SLE with different disease activity. The disease activity of SLE was measured by Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K). The disease activity was further classified as: lower disease activity SLEDAI-2K < 3 and higher disease activity SLEDAI-2K ≥ 3. After adjusting for age and sex, T cells from SLE patients with higher disease activity still had lower expression levels of circ-CAMTA1 ( P < 0.001), circ-ASH1L ( P = 0.009), and circ-ASAP1 ( P < 0.001) compared to those from SLE patients with low disease activity. Data was presented as mean ± SD. (ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001)

Article Snippet: To analyzed circRNA expression levels in Jurkat cells after stimulation, cells were treated with phorbol 12-myristate 13-acetate (PMA; 20 ng/mL; Sigma-Aldrich) and ionomycin (500 ng/mL; Sigma-Aldrich, ) for 24 h. For analysis of circRNA expression in response to interferon-alpha (IFN-α), Jurkat cells were treated with 1000 IU/mL recombinant human IFN-α for 72 h. The IFN-alpha we used was purchased from Proteintech (Rosemont, IL, USA) and was produced in HEK293.

Techniques: Quantitative Proteomics, Activity Assay, Expressing, Control

Journal: Arthritis Research & Therapy

Article Title: Circ-CAMTA1 regulated by Ca 2+ influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosus

doi: 10.1186/s13075-024-03422-6

Figure Lengend Snippet: Regression analyses assessing the correlations between the expression levels of circRNA transcripts with demographic data of patients with systemic lupus erythematosus

Article Snippet: To analyzed circRNA expression levels in Jurkat cells after stimulation, cells were treated with phorbol 12-myristate 13-acetate (PMA; 20 ng/mL; Sigma-Aldrich) and ionomycin (500 ng/mL; Sigma-Aldrich, ) for 24 h. For analysis of circRNA expression in response to interferon-alpha (IFN-α), Jurkat cells were treated with 1000 IU/mL recombinant human IFN-α for 72 h. The IFN-alpha we used was purchased from Proteintech (Rosemont, IL, USA) and was produced in HEK293.

Techniques: Expressing, Activity Assay